The effects of time-restricted eating and Ramadan fasting on gut microbiota composition: a systematic review of human and animal studies
Joanna Maria Pieczyńska-Zając 1, Anna Malinowska 2, Karolina Łagowska 1, Natalia Leciejewska 3, Joanna Bajerska 1
1Department of Human Nutrition and Dietetics, Poznań University of Life Sciences, Poznań, Poland.
2Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.
3Department of Physiology, Biochemistry, and Biostructure of Animals, Poznań University of Life Sciences, Poznań, Poland.
Context: It is well known that the microbiome undergoes cyclical diurnal rhythms. It has thus been hypothesized that meal timing may affect gut microbial composition, function, and host health.
Objective: This review aims to examine the effects of time-restricted eating (TRE) and Ramadan fasting (RF) on the composition of the gut microbiota in animal and human studies. The associations between composition of microbiota and host metabolic parameters are also examined.
Data sources: A search was performed on the PubMed, Cochrane, Scopus, and Web of Science databases up to December 31, 2022. The search strategy was performed using the Medical Subject Heading (MeSH) terms “intermittent fasting” and “gastrointestinal microbiome” and the key words “Ramadan fasting” and “microbes.”
Data extraction: Seven human studies (4 TRE and 3 RF) and 9 animal studies (7 TRE, 2 RF-like) were retrieved.
Data analysis: TRE and RF in human studies lead to an increase in gut microbial community alpha-diversity. In animal studies (both TRE and RF-like), fasting is not associated with improved alpha-diversity, but enhancement of microbial fluctuation is observed, compared with high-fat diet ad libitum groups. Within Firmicutes and Bacteroidetes phyla, no specific direction of changes resulting from fasting are observed in both animals and human. After TRE or RF, a greater abundance of the Faecalibacterium genus is observed in human studies; changes in Lactobacillus abundance are found in animal studies; and increases in Akkermansia are seen both in humans and in animals fed a feed-pellet diet. Only 2 human studies show a beneficial correlation between microbiota changes and host metabolic (HDL cholesterol) or anthropometric parameters (body mass index).
Conclusions: These findings support the importance of both regimens in improving the gut microbiota composition. However, based on results of animal studies, it can be suggested that diet remains the essential factor in forming the microbiota’s environment.
Systematic review registration: PROSPERO registration no. CRD42021278918.
Keywords: chrononutrition; fasting; gut microbiota; host health; microbial diversity.
