Maternal cafeteria diet influences kisspeptin (Kiss1), kisspeptin receptor(Gpr54), and sirtuin (Sirt1) genes, hormonal and metabolic profiles, and reproductive functions in rat offspring in a sex-specific manner
Julia Matuszewska, Joanna Nowacka-Woszuk, Anna Radziejewska, Emilia Grzęda, Ewa Pruszynska-Oszmałek, Łukasz Dylewski, Agata Chmurzynska, Joanna H Sliwowska
Kisspeptin (KP, encoded by Kiss1, binding to the Gpr54 receptor) is a neuropeptide conveying information on the metabolic status to the hypothalamic-pituitary-gonadal (HPG) axis. KP acts together with dynorphin A (DYN A, encoded by Pdyn) and neurokinin B (NKB, encoded by Tac2) to regulate reproduction. Moreover, KP is crucial for the onset of puberty and is under the control of sirtuin (encoded by Sirt1).We hypothesize that the maternal cafeteria (CAF) diet has adverse effects on the offspring’s hormonal, metabolic and reproductive functions due to sex-specific alterations in the expression of Kiss1, Gpr54, Pdyn, Tac2, and Sirt1 in the hypothalamus, and Kiss1, Gpr54, Sirt1 in the liver.Rats were fed a CAF diet before pregnancy, during pregnancy and lactation. The vaginal opening was monitored. Offspring were sacrificed in three age points: PND 30, PND 35 and PND 60 (females) and PND 40, PND 45 and PND 60 (males). Their metabolic and hormonal status was assessed. mRNA for Kiss1, Gpr54, Pdyn, Tac2 and Sirt1 were measured by real-time PCR in the hypothalamus and/or livers. We found that CAF offspring had: lower weight and altered body composition; increased cholesterol and triglycerides levels, sex-specific changes in glucose and insulin levels; sex-dependent changes in Sirt1/Kiss1 mRNA ratio in the hypothalamus, sex-specific alterations in Kiss1 and Sirt1 mRNA in the liver with more diversity in males; a delayed puberty onset in females. We concluded that the mother’s CAF diet leads to sex-specific alterations in metabolic and reproductive outcomes via Kiss1/Gpr54 and Sirt1 systems in offspring.
Biology of Reproduction, 2023;, ioad101, https://doi.org/10.1093/biolre/ioad101
Key words: obesity, puberty, cafeteria diet, kisspeptin, KNDy neurons, liver, hypothalamus, 28 SIRT1
